5C4K
APH(2")-IVa in complex with GET (G418) at room temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 291.15 |
| Detector technology | CCD |
| Collection date | 2015-05-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9797 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.703, 64.941, 78.870 |
| Unit cell angles | 90.00, 90.73, 90.00 |
Refinement procedure
| Resolution | 78.860 - 3.050 |
| R-factor | 0.1612 |
| Rwork | 0.159 |
| R-free | 0.19920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4n57 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.515 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.440 | 3.050 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.087 | 0.529 |
| Number of reflections | 16269 | |
| <I/σ(I)> | 2.6 | 1.4 |
| Completeness [%] | 90.4 | 93.4 |
| Redundancy | 1.6 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 291.15 | 12% PEG3350, 50-90 mM Ammonium citrate |
