5C3O

Crystal structure of the C-terminal truncated Neurospora crassa T7H (NcT7HdeltaC) in apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-01-08
Detector	RAYONIX MX225HE
Wavelength(s)	0.9792
Spacegroup name	P 63
Unit cell lengths	119.148, 119.148, 56.631
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.300
R-factor	0.1978
Rwork	0.196
R-free	0.22410
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.054
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SADABS
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.380
High resolution limit [Å]	2.300	4.950	2.300
Rmerge	0.102	0.057	0.540
Total number of observations	217111
Number of reflections	20591
<I/σ(I)>	11.5		5
Completeness [%]	100.0	99.8	100
Redundancy	10.5	10	10.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	0.2 M (NH4)2SO4, 0.1 M MES, 30%(W/v) PEGMME 5000