5C33

Crystal Structure of Mouse Ryanodine Receptor 2 SPRY1 Domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.975910, 0.979518, 1.377552
Spacegroup name	P 21 21 21
Unit cell lengths	53.246, 64.085, 109.769
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.371 - 1.210
R-factor	0.1447
Rwork	0.143
R-free	0.17250
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.594
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.371	1.250
High resolution limit [Å]	1.210	1.210
Rmerge	0.058	0.915
Number of reflections	114712
<I/σ(I)>	17.81	2.16
Completeness [%]	99.9	98.8
Redundancy	7	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	277	0.1 M sodium acetate, pH 5.0, 0.6-0.9 M sodium sulfate