5C30
Crystal Structure of Rabbit Ryanodine Receptor 1 Repeat12 Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-02 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 94.440, 94.440, 67.220 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.220 - 1.550 |
| R-factor | 0.1497 |
| Rwork | 0.149 |
| R-free | 0.16450 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.355 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.220 | 1.605 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.123 | 1.509 |
| Number of reflections | 50032 | |
| <I/σ(I)> | 13.82 | 2.17 |
| Completeness [%] | 99.2 | 98.14 |
| Redundancy | 11.9 | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 0.1 M HEPES, pH 7.0, 19% w/v PEG20000 |
