5C2Y

Crystal structure of the Saccharomyces cerevisiae Rtr1 (regulator of transcription)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-15
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.288
Spacegroup name	P 43 21 2
Unit cell lengths	119.009, 119.009, 69.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.181 - 2.600
R-factor	0.2123
Rwork	0.207
R-free	0.26240
RMSD bond length	0.009
RMSD bond angle	1.212
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.600	2.600
Number of reflections	14886
<I/σ(I)>	9.24	1.32
Completeness [%]	93.6	71.2
Redundancy	5.6	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	0.1M HEPES pH 7.5, 1.25M Lithium sulfate