5C2U

Ferredoxin-like domain of nucleoporin Nup54 bound to a nanobody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-24
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.000
Spacegroup name	P 31 2 1
Unit cell lengths	90.860, 90.860, 57.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.741 - 1.550
R-factor	0.1779
Rwork	0.176
R-free	0.20490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4klm
RMSD bond length	0.008
RMSD bond angle	1.051
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.500		1.580
High resolution limit [Å]	1.550	6.710	1.540
Rmerge	0.065	0.043	1.665
Rmeas	0.067	0.045	1.750
Total number of observations	672687
Number of reflections	40071	548	3107
<I/σ(I)>	22.9	61.12	1.52
Completeness [%]	99.9	99.8	99.3
Redundancy	15.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	PEG 6000, Sodium acetate trihydrate