5C2G
GWS1B RubisCO: Form II RubisCO derived from uncultivated Gallionellacea species (CABP-bound).
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2015-05-27 |
Detector | RIGAKU RAXIS HTC |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.380, 120.930, 161.440 |
Unit cell angles | 90.00, 90.54, 90.00 |
Refinement procedure
Resolution | 40.358 - 2.597 |
R-factor | 0.183 |
Rwork | 0.181 |
R-free | 0.22190 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.017 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.360 | 2.660 | |
High resolution limit [Å] | 2.597 | 11.610 | 2.597 |
Rmerge | 0.243 | 0.064 | 0.891 |
Rmeas | 0.261 | 0.068 | 0.960 |
Total number of observations | 661495 | ||
Number of reflections | 86719 | 1017 | 5718 |
<I/σ(I)> | 9.06 | 25.82 | 2.29 |
Completeness [%] | 98.3 | 97.3 | 87.7 |
Redundancy | 7.6 | 7.09 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | protein buffer: 20 mM Tris, pH 8.0, 300 mM NaCl, 10% glycerol, 10 mM MgCl2, 20 mM NaHCO3. Protein concentration: 16 mg/ml. Crystals grew using a 1:1 ratio of protein to reservoir solution (160 mM calcium acetate, 80 mM sodium cacodylate pH 6.5, 14.4% PEG 8000, 20% glycerol). |