5C1Q
Serine/threonine-protein kinase pim-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2014-10-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 65 |
| Unit cell lengths | 99.370, 99.370, 80.680 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.860 - 3.000 |
| R-factor | 0.17764 |
| Rwork | 0.174 |
| R-free | 0.24575 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.704 |
| Data scaling software | DENZO |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.860 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 9182 | |
| <I/σ(I)> | 19.2 | 6.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.6 | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 291 | 0.4 M potassium sodium tartrate tetrahydrate |
