5C1I
m1A58 tRNA methyltransferase mutant - D170A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-09-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.980 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 110.429, 110.429, 306.441 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 51.940 - 3.100 |
| Rwork | 0.200 |
| R-free | 0.26860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pwy |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER (2.5.1) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 95.635 | 95.635 | 3.270 |
| High resolution limit [Å] | 3.100 | 9.800 | 3.100 |
| Rmerge | 0.027 | 0.686 | |
| Rmeas | 0.136 | ||
| Rpim | 0.052 | 0.013 | 0.282 |
| Total number of observations | 135340 | 4374 | 20186 |
| Number of reflections | 21045 | ||
| <I/σ(I)> | 9.6 | 20.8 | 2.4 |
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 6.4 | 5.3 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 290 | 2.4 M ammonium sulfate and 10%, isopropanol (v/v) |
