5C1F

Structure of the Imp2 F-BAR domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-28
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.00
Spacegroup name	C 1 2 1
Unit cell lengths	187.337, 33.802, 120.765
Unit cell angles	90.00, 122.54, 90.00

Refinement procedure

Resolution	19.968 - 2.355
R-factor	0.1929
Rwork	0.190
R-free	0.23910
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.531
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.430
High resolution limit [Å]	2.350	2.350
Rmerge	0.099	0.354
Number of reflections	26635
<I/σ(I)>	11.8	2.6
Completeness [%]	98.5	88.2
Redundancy	3.3	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	18% PEG 3350, 0.2 M AmFormate
1	VAPOR DIFFUSION, HANGING DROP	8	298	18% PEG 3350, 0.2 M AmFormate