5C15
K428A mutant nuclease domain of the large terminase subunit gp2 of bacterial virus Sf6 with Manganese
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-09-09 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.03316 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 132.766, 57.365, 46.646 |
Unit cell angles | 90.00, 98.71, 90.00 |
Refinement procedure
Resolution | 18.922 - 1.570 |
R-factor | 0.1647 |
Rwork | 0.164 |
R-free | 0.18600 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.173 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.600 |
High resolution limit [Å] | 1.570 | 1.570 |
Rmerge | 0.040 | 0.770 |
Number of reflections | 47321 | |
<I/σ(I)> | 27 | 1 |
Completeness [%] | 97.6 | 84.5 |
Redundancy | 4.1 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100mM HEPES, pH 7.5, 50mM NaCl, 8% PEG8000 |