5C0V
Structure of the LARP1-unique domain DM15
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2013-08-16 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.730, 61.461, 83.202 |
| Unit cell angles | 90.00, 115.53, 90.00 |
Refinement procedure
| Resolution | 42.567 - 2.200 |
| R-factor | 0.177 |
| Rwork | 0.176 |
| R-free | 0.22160 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.005 |
| Phasing software | PHENIX (1.8.2_1309) |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.096 | 0.090 |
| Number of reflections | 35696 | |
| <I/σ(I)> | 34.2 | 2.25 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 295 | 0.2 M Ammonium nitrate, 20% PEG 3350 |
