5C0G
HLA-A02 carrying YLGGPDFPTI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.030, 79.170, 58.290 |
| Unit cell angles | 90.00, 115.54, 90.00 |
Refinement procedure
| Resolution | 43.808 - 1.370 |
| R-factor | 0.1641 |
| Rwork | 0.163 |
| R-free | 0.18760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3utq |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.237 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.808 | 43.808 | 1.410 |
| High resolution limit [Å] | 1.370 | 6.130 | 1.370 |
| Rmerge | 0.024 | 0.894 | |
| Rmeas | 0.067 | ||
| Rpim | 0.035 | 0.016 | 0.602 |
| Total number of observations | 325767 | 4205 | 22545 |
| Number of reflections | 91447 | ||
| <I/σ(I)> | 11.6 | 29.2 | 1.9 |
| Completeness [%] | 96.9 | 99.4 | 95.1 |
| Redundancy | 3.6 | 3.8 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M sodium nitrate, 0.1M BIS TRIS propane pH6.5, 20% PEG 3350 |
