5BZA

Crystal structure of CbsA from Thermotoga neapolitana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-30
Detector	MAR CCD 165 mm
Wavelength(s)	0.9
Spacegroup name	H 3 2
Unit cell lengths	158.965, 158.965, 517.242
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.741 - 2.002
R-factor	0.2384
Rwork	0.238
R-free	0.27630
RMSD bond length	0.013
RMSD bond angle	1.485
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.000
Number of reflections	158414
<I/σ(I)>	9.25
Completeness [%]	94.2
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287	0.1 M HEPES pH 7.5 1.0 M sodium acetate 0.05 M CdCl2