5BZ7
X-ray crystal structure of a continuously hydrogen bonded 14mer DNA lattice.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 77.15 |
| Detector technology | PIXEL |
| Collection date | 2014-06-29 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.979200 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 26.013, 26.013, 122.022 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.530 - 2.030 |
| R-factor | 0.23875 |
| Rwork | 0.234 |
| R-free | 0.29340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5bxw |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.567 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.530 | 2.090 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.111 | 0.979 |
| Number of reflections | 3436 | |
| <I/σ(I)> | 13.9 | 1 |
| Completeness [%] | 99.4 | 97.3 |
| Redundancy | 10.3 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 296.15 | 120mM Magnesium Formate 50mM Lithium Chloride 10% MPD |
