5BZ6

Crystal structure of the N-terminal domain single mutant (S92A) of the human mitochondrial calcium uniporter fused with T4 lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2013-09-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 65
Unit cell lengths	97.803, 97.803, 61.537
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.890 - 2.750
R-factor	0.167
Rwork	0.164
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2lzm 4xsj
RMSD bond length	0.014
RMSD bond angle	1.670
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.750
Number of reflections	11171
<I/σ(I)>	12.7
Completeness [%]	99.5
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	25% PEG 3350, 0.1M Bis-Tris-HCl (pH 6.5), 0.2M ammonium sulfate