5BYP

Crystal structure of an uncharacterized protein (BACEGG_01585) from Bacteroides eggerthii DSM 20697 at 2.60 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-05-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97839,0.91837,0.9798
Spacegroup name	I 41 2 2
Unit cell lengths	64.800, 64.800, 235.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.712 - 2.600
R-factor	0.2495
Rwork	0.247
R-free	0.29980
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	0.830
Data scaling software	XDS
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.712	42.712	2.640
High resolution limit [Å]	2.600	5.460	2.550
Rmerge	0.043	0.026	1.054
Rmeas	0.047	0.028	1.175
Total number of observations	73713
Number of reflections	8129	1603	584
<I/σ(I)>	20.71	49.3	1.2
Completeness [%]	93.4	99.3	37.8
Redundancy	5.033

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	3.5	293	34.00% polyethylene glycol 6000, 0.100M Sodium Iodide, 0.1M sodium citrate - citric acid pH 3.5