5BYM

Crystal structure of the RNA-binding domain of yeast Puf5p bound to SMX2 RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-05
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	94.215, 100.792, 49.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.676 - 2.708
R-factor	0.2461
Rwork	0.241
R-free	0.28530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5bym
RMSD bond length	0.004
RMSD bond angle	0.712
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.700	2.700
Rmerge	0.049	0.239
Number of reflections	12843
<I/σ(I)>	38	6.2
Completeness [%]	95.9	73.9
Redundancy	6.8	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	293	15% PEG3350, 0.1M CTBP