5BYD
Crystal structure of human ribokinase in P21 spacegroup
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.590, 89.910, 75.140 |
| Unit cell angles | 90.00, 98.80, 90.00 |
Refinement procedure
| Resolution | 44.960 - 2.100 |
| R-factor | 0.17377 |
| Rwork | 0.171 |
| R-free | 0.21536 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fv7 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.735 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.960 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.083 | 0.518 |
| Number of reflections | 34784 | |
| <I/σ(I)> | 8.7 | 2.2 |
| Completeness [%] | 97.4 | 93.4 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1M sodium HEPES, 25% PEG 6000 |
