5BY8
The structure of Rpf2-Rrs1 explains its role in ribosome biogenesis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-04 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.968 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 49.160, 84.140, 194.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.446 - 1.515 |
| R-factor | 0.1478 |
| Rwork | 0.145 |
| R-free | 0.19080 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.324 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.450 | 1.569 |
| High resolution limit [Å] | 1.515 | 1.515 |
| Rmerge | 0.070 | 1.220 |
| Number of reflections | 61799 | |
| <I/σ(I)> | 11.6 | 0.7 |
| Completeness [%] | 98.0 | 84.1 |
| Redundancy | 4.8 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M KCl and 20% (w/v) PEG 3350 |
