5BY1
H18 Bat Influenza NS1 RNA Binding Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-20 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.661, 56.834, 39.814 |
| Unit cell angles | 90.00, 97.95, 90.00 |
Refinement procedure
| Resolution | 19.716 - 1.751 |
| R-factor | 0.1649 |
| Rwork | 0.160 |
| R-free | 0.20860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ail |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.996 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.780 |
| High resolution limit [Å] | 1.750 | 4.740 | 1.750 |
| Rmerge | 0.058 | 0.052 | 0.416 |
| Total number of observations | 41172 | ||
| Number of reflections | 13038 | ||
| <I/σ(I)> | 14.6 | ||
| Completeness [%] | 94.9 | 97.5 | 57.8 |
| Redundancy | 3.2 | 3.2 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 292 | 0.2M Na Acetate, 0.1M MES pH 6.5, 30% PEG400 |
