5BW5
Crystal structure of the 16S rRNA (adenine(1408)-N(1))-methyltransferase D21A mutant from Catenulisporales acidiphilia
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-07 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.946, 66.663, 82.608 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.103 - 2.500 |
R-factor | 0.2444 |
Rwork | 0.239 |
R-free | 0.29250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4x1o |
RMSD bond length | 0.003 |
RMSD bond angle | 0.736 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.320 | |
Number of reflections | 8687 | |
<I/σ(I)> | 15.8 | 2.4 |
Completeness [%] | 97.9 | 86 |
Redundancy | 3.5 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.2 M Ammonium Acetate, 0.1 M Sodium Acetate trihydrate, pH 4.6, 30% PEG4000 |