5BW0
The crystal structure of minor pseudopilin binary complex of XcpV and XcpW from the Type 2 secretion system of Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-08-22 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.110, 200.950, 66.450 |
| Unit cell angles | 90.00, 95.14, 90.00 |
Refinement procedure
| Resolution | 19.700 - 2.000 |
| R-factor | 0.2089 |
| Rwork | 0.206 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ret |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.098 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 19.700 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.110 |
| Number of reflections | 68807 |
| <I/σ(I)> | 11.73 |
| Completeness [%] | 97.9 |
| Redundancy | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 10-15% PEG 3350, 0.1M Tris, pH 7.0, 0.5M caesium chloride . Protein solution: 25mM HEPES, pH 7.0, 150mM sodium chloride |
