5BV9
The Structure of Bacillus pumilus GH48 in complex with cellobiose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-18 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 97.291, 97.291, 218.108 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.930 |
| R-factor | 0.1636 |
| Rwork | 0.161 |
| R-free | 0.20747 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g9g |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.912 |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | PROTEUM PLUS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Number of reflections | 79431 | |
| <I/σ(I)> | 9.58 | 2.03 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 8.72 | 3.93 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 2.4 M Sodium malonate, pH 5.0 |
