5BUV
X-ray structure of WbcA from Yersinia enterocolitica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-17 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 79.160, 42.678, 150.463 |
| Unit cell angles | 90.00, 90.39, 90.00 |
Refinement procedure
| Resolution | 27.360 - 1.750 |
| R-factor | 0.19513 |
| Rwork | 0.192 |
| R-free | 0.24795 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hn0 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.998 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.341 | |
| Number of reflections | 50180 | |
| <I/σ(I)> | 6.9 | 2 |
| Completeness [%] | 98.1 | 95 |
| Redundancy | 2.5 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 14-18% PEG5000-OMe, 5 mM CPD-6-deoxygulose, 2% glycerol, 100 mM MOPS |
