5BUP
Crystal structure of the ZP-C domain of mouse ZP2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97939 |
| Spacegroup name | P 61 |
| Unit cell lengths | 105.210, 105.210, 40.550 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.050 - 2.251 |
| R-factor | 0.2042 |
| Rwork | 0.201 |
| R-free | 0.22830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.859 |
| Data reduction software | XDS (20141103) |
| Data scaling software | XDS |
| Phasing software | PHASER (2.5.7) |
| Refinement software | PHENIX (dev-1924) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.557 | 2.332 |
| High resolution limit [Å] | 2.251 | 2.251 |
| Rmerge | 0.102 | |
| Number of reflections | 12293 | |
| <I/σ(I)> | 9.74 | 1.19 |
| Completeness [%] | 95.0 | 100 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 25% PEG 3350, 0.1 M sodium acetate pH 5.5, 0.2 M ammonium sulfate, 20% glycerol |
