5BUL
Structure of flavin-dependent brominase Bmp2 triple mutant Y302S F306V A345W
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.232, 51.531, 77.062 |
| Unit cell angles | 90.00, 100.89, 90.00 |
Refinement procedure
| Resolution | 32.974 - 1.978 |
| R-factor | 0.1806 |
| Rwork | 0.179 |
| R-free | 0.21450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5bva |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.085 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 1.978 |
| Rmerge | 0.062 |
| Number of reflections | 26147 |
| <I/σ(I)> | 30.01 |
| Completeness [%] | 98.8 |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG |
