5BU8
HK620 Tail Needle crystallized at pH 7.5 and derivatized with Xenon
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2007-05-06 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 3 |
| Unit cell lengths | 43.642, 43.642, 271.063 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.988 - 2.202 |
| R-factor | 0.2306 |
| Rwork | 0.226 |
| R-free | 0.25070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2poh |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.892 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.988 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.100 | |
| Number of reflections | 26538 | |
| <I/σ(I)> | 23.5 | 7.9 |
| Completeness [%] | 90.5 | 58.5 |
| Redundancy | 4.2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 30% PEG 1000, 0.1M Tris pH 7.5 derivatized with Xenon gas prior to data collection |
