5BU7
Crystal structure of an engineered protein that forms nanotubes with tunable diameters
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2013-01-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 52.890, 52.890, 257.080 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.800 - 2.460 |
| R-factor | 0.2284 |
| Rwork | 0.225 |
| R-free | 0.29230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.385 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 85.690 |
| High resolution limit [Å] | 2.460 |
| Number of reflections | 8104 |
| <I/σ(I)> | 6.7 |
| Completeness [%] | 95.1 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7.5 | 298 | 50-fold excess of zinc chloride |
