5BU1
Structure of the truncated C-terminal domain of lpg1496 from Legionella pneumophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-13 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9770 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.888, 71.466, 76.996 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.610 - 1.600 |
| R-factor | 0.16231 |
| Rwork | 0.160 |
| R-free | 0.20381 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5bu0 |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.591 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.465 | |
| Number of reflections | 42595 | |
| <I/σ(I)> | 34.2 | 8.5 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 14.4 | 14.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.1 M sodium malonate, 0.1 M HEPES, 0.5% (v/v) Jeffamine ED-2001 |
