5BU0
Structure of the C-terminal domain of lpg1496 from Legionella pneumophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9770 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.976, 77.232, 109.378 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.931 - 2.350 |
| R-factor | 0.2418 |
| Rwork | 0.239 |
| R-free | 0.29330 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.651 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.446 | |
| Number of reflections | 25991 | |
| <I/σ(I)> | 42.5 | 11.4 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 13 | 12.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 0.1 M Bicine, 20% (w/v) PEG 6000 |
