5BTY
Structure of the middle domain of lpg1496 from Legionella pneumophila in P21 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-08 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.802, 35.910, 43.661 |
| Unit cell angles | 90.00, 104.67, 90.00 |
Refinement procedure
| Resolution | 40.470 - 1.150 |
| R-factor | 0.17055 |
| Rwork | 0.169 |
| R-free | 0.19218 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.122 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.170 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.437 | |
| Number of reflections | 39061 | |
| <I/σ(I)> | 43.2 | 3.1 |
| Completeness [%] | 92.0 | 57.9 |
| Redundancy | 7.4 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 25% PEG 3350, 0.1 M HEPES |
