5BTX
Structure of the N-terminal domain of lpg1496 from Legionella pneumophila in complex with nucleotide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-06 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9770 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.622, 57.446, 63.779 |
| Unit cell angles | 90.00, 93.32, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.2404 |
| Rwork | 0.238 |
| R-free | 0.29159 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5btw |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.643 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.497 | |
| Number of reflections | 12544 | |
| <I/σ(I)> | 39 | 5.3 |
| Completeness [%] | 89.2 | 92.3 |
| Redundancy | 6.6 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M sodium chloride, 0.1 M Bis-Tris pH6.5, 25% (w/v) PEG3350 |
