5BTP

Fusobacterium ulcerans ZTP riboswitch bound to ZMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 32 2 1
Unit cell lengths	92.661, 92.661, 121.172
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.330 - 2.816
R-factor	0.1892
Rwork	0.186
R-free	0.22060
RMSD bond length	0.002
RMSD bond angle	0.829
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.330
High resolution limit [Å]	2.816
Rmerge	0.114
Number of reflections	14947
<I/σ(I)>	26.1
Completeness [%]	99.5
Redundancy	10.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	298	LiSO4 and PEG 4000