5BTM
Crystal structure of AUUCU repeating RNA that causes spinocerebellar ataxia type 10 (SCA10)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 41 |
| Unit cell lengths | 63.120, 63.120, 72.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.120 - 2.778 |
| R-factor | 0.17829 |
| Rwork | 0.176 |
| R-free | 0.22425 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.675 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 75.080 |
| High resolution limit [Å] | 2.778 |
| Number of reflections | 7995 |
| <I/σ(I)> | 20.9 |
| Completeness [%] | 100.0 |
| Redundancy | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 296 | 100 mM ammonium acetate, 5 mM Mg sulfate, 50 mM MES pH 6.0, and 600 mM NaCl |
