5BT1

histone chaperone Hif1 playing with histone H2A-H2B dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	C 1 2 1
Unit cell lengths	102.673, 48.363, 160.706
Unit cell angles	90.00, 107.05, 90.00

Refinement procedure

Resolution	43.380 - 2.620
R-factor	0.2104
Rwork	0.207
R-free	0.26180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nq0
RMSD bond length	0.008
RMSD bond angle	1.049
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.670
High resolution limit [Å]	2.620	7.110	2.620
Rmerge	0.105	0.033	0.626
Rmeas	0.123	0.038	0.734
Rpim	0.062	0.019	0.375
Total number of observations	80873
Number of reflections	22738
<I/σ(I)>	7.4
Completeness [%]	98.5	95.8	98.7
Redundancy	3.6	3.6	3.5
CC(1/2)		0.998	0.683

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287	0.1M Magnesium formate dihydrate, 15% PEG3350