5BSB
Lipomyces starkeyi levoglucosan kinase bound to levoglucosan
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-10-02 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97949 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 70.320, 70.320, 263.334 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.860 - 1.850 |
R-factor | 0.1949 |
Rwork | 0.194 |
R-free | 0.21920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4yh5 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.035 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.860 | 1.950 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.096 | 0.861 |
Number of reflections | 57761 | |
<I/σ(I)> | 9.8 | 1.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 5.6 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 296 | Equal amounts of LGK (9 mg/ml) in 50 mM NaCl, 0.5 mM TCEP, 20 mM Tris pH 7.5 and 200 mM levoglucosan were mixed with reservoir buffer containing 1.8 M ammonium sulfate, 0.1 M HEPES 7.0 and 0.1 M sodium acetate. |