5BSA

Structure of histone H3/H4 in complex with Spt2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 43 2 2
Unit cell lengths	128.351, 128.351, 116.814
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.115 - 4.611
R-factor	0.243
Rwork	0.239
R-free	0.32890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1aoi
RMSD bond length	0.013
RMSD bond angle	1.972
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXDE
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	4.680
High resolution limit [Å]	4.600	4.600
Rmerge	0.066
Number of reflections	5704
<I/σ(I)>	22.3	1.3
Completeness [%]	98.7	88.6
Redundancy	11.4	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	293	0.02 M NaCl, 0.2 M HEPES 7.5, 1.6 M ammonium sulfate