5BS2

Crystal structure of RbcX-IIa from Chlamydomonas reinhardtii in complex with RbcL C-terminal tail

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-05-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99988
Spacegroup name	P 1
Unit cell lengths	34.217, 38.525, 50.355
Unit cell angles	88.47, 81.53, 67.92

Refinement procedure

Resolution	30.000 - 1.970
R-factor	0.2007
Rwork	0.200
R-free	0.22190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5bs1
RMSD bond length	0.013
RMSD bond angle	1.423
Data reduction software	XDS
Data scaling software	SCALA (3.3.20)
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.781	35.683	2.070
High resolution limit [Å]	1.970	6.220	1.970
Rmerge		0.034	0.494
Rmeas	0.102
Rpim	0.060	0.028	0.394
Total number of observations	38750	1228	4818
Number of reflections	15820
<I/σ(I)>	10.4	26.5	2
Completeness [%]	94.2	91	87.9
Redundancy	2.4	2.5	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M Tris-HCl pH 8.5, 25% PEG2000 MME