5BRQ
Crystal structure of Bacillus licheniformis trehalose-6-phosphate hydrolase (TreA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.903 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.802, 97.584, 108.336 |
| Unit cell angles | 98.20, 91.44, 108.15 |
Refinement procedure
| Resolution | 24.304 - 2.003 |
| R-factor | 0.1817 |
| Rwork | 0.181 |
| R-free | 0.24530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uok |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.114 |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 149079 |
| <I/σ(I)> | 29 |
| Completeness [%] | 96.8 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 20% (w/v) PEG 3350, 0.2 M magnesium acetate hexahydrate, 2% Tacsimate (pH 5.0) |
