5BR9

Crystal structure of an uncharacterized protein with similarity to peptidase YEAZ from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-12
Detector	RAYONIX MX-225
Wavelength(s)	0.97872
Spacegroup name	C 2 2 21
Unit cell lengths	80.350, 201.940, 162.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.222 - 2.350
R-factor	0.1632
Rwork	0.161
R-free	0.20570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3r6m
RMSD bond length	0.007
RMSD bond angle	1.070
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	BALBES
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		2.410
High resolution limit [Å]	2.350	10.510	2.350
Rmerge	0.071	0.026	0.537
Rmeas	0.079	0.029	0.600
Total number of observations	277630
Number of reflections	55353	667	4045
<I/σ(I)>	16.65	42.93	2.98
Completeness [%]	99.7	94.1	100
Redundancy	5		5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	Microlytics MCSG1 A3: 10% PEG 8000, 200mM NaCl, 100mM Na2HPO4/ KH2PO4 pH 6.2; PsaeA.17385.a.B1.PS02242 at 20mg/ml; cryo: 20% EG in 2 steps; tray 260042a3, puck ges3-7