5BQT
Structure of TrmBL2, an archaeal chromatin protein, shows a novel mode of DNA binding.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-29 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 82.298, 235.143, 63.511 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.836 - 3.000 |
| R-factor | 0.2355 |
| Rwork | 0.233 |
| R-free | 0.28880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5bpd |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.108 |
| High resolution limit [Å] | 3.000 | 3.001 |
| Rmerge | 0.110 | |
| Number of reflections | 25362 | |
| <I/σ(I)> | 10.99 | |
| Completeness [%] | 99.6 | 98 |
| Redundancy | 6.3 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.2M Calcium acetate and 21% PEG 3350 |
