5BQK
CRYSTAL STRUCTURE OF C-TERMINAL DOMAIN OF ICP27 PROTEIN FROM HSV-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97893 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 156.983, 120.335, 79.037 |
| Unit cell angles | 90.00, 100.46, 90.00 |
Refinement procedure
| Resolution | 23.795 - 2.000 |
| R-factor | 0.2185 |
| Rwork | 0.217 |
| R-free | 0.24870 |
| Structure solution method | SAD |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.829 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.795 | 2.068 |
| High resolution limit [Å] | 1.997 | 1.997 |
| Number of reflections | 89418 | |
| <I/σ(I)> | 1.46 | |
| Completeness [%] | 91.5 | 93.91 |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | Sodium Thiocyanate, Benzamidine Hydrochloride |
