5BPZ

Atomic-resolution structures of the APC/C subunits Apc4 and the Apc5 N-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	CCD
Collection date	2013-05-01
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0081
Spacegroup name	P 21 21 2
Unit cell lengths	40.972, 57.297, 63.616
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.000 - 2.180
R-factor	0.2122
Rwork	0.211
R-free	0.22630
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	BUSTER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.630	2.240
High resolution limit [Å]	2.180	2.180
Rmerge	0.055	0.663
Number of reflections	8213
<I/σ(I)>	28.5	4.5
Completeness [%]	99.8	99.1
Redundancy	12.8	12.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	The protein was concentrated to 3.4 mg/mL. Plate-like crystals were grown in a buffer containing 40 mM sodium propionate, 20 mM sodium cacodylate, 40 mM Bis-Tris propane (pH 7.0) and 25% PEG1500. Larger crystals were obtained by seeding.