5BPV
Crystal Structure of Zaire ebolavirus VP35 RNA binding domain mutant I278A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-03 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.542 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.750, 65.660, 72.020 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 13.905 - 1.952 |
R-factor | 0.1821 |
Rwork | 0.180 |
R-free | 0.21840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fke |
RMSD bond length | 0.007 |
RMSD bond angle | 1.030 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA (3.3.20) |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.522 | 13.905 | 2.060 |
High resolution limit [Å] | 1.952 | 6.170 | 1.950 |
Rmerge | 0.063 | 0.281 | |
Rmeas | 0.113 | ||
Rpim | 0.043 | 0.027 | 0.135 |
Total number of observations | 122680 | 3877 | 13760 |
Number of reflections | 18397 | ||
<I/σ(I)> | 11.8 | 19.3 | 4.8 |
Completeness [%] | 99.7 | 91.6 | 99.7 |
Redundancy | 6.7 | 6.4 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2 M potassium sodium tartrate tetrahydrate, 20% PEG 3350 |