5BPV

Crystal Structure of Zaire ebolavirus VP35 RNA binding domain mutant I278A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-03
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.542
Spacegroup name	P 21 21 21
Unit cell lengths	51.750, 65.660, 72.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	13.905 - 1.952
R-factor	0.1821
Rwork	0.180
R-free	0.21840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fke
RMSD bond length	0.007
RMSD bond angle	1.030
Data reduction software	iMOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.522	13.905	2.060
High resolution limit [Å]	1.952	6.170	1.950
Rmerge		0.063	0.281
Rmeas	0.113
Rpim	0.043	0.027	0.135
Total number of observations	122680	3877	13760
Number of reflections	18397
<I/σ(I)>	11.8	19.3	4.8
Completeness [%]	99.7	91.6	99.7
Redundancy	6.7	6.4	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2 M potassium sodium tartrate tetrahydrate, 20% PEG 3350