5BPQ

Crystal structure of the cysteine-rich domain of human Frizzled 4 - Crystal Form II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9686
Spacegroup name	P 61
Unit cell lengths	76.075, 76.075, 204.470
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	65.880 - 2.400
R-factor	0.2058
Rwork	0.204
R-free	0.24290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5bpb
RMSD bond length	0.005
RMSD bond angle	1.075
Data reduction software	xia2
Data scaling software	Aimless (0.1.29)
Phasing software	PHASER (2.5.6)
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.370	47.370	2.490
High resolution limit [Å]	2.400	8.980	2.400
Rmerge	0.046	0.022	0.606
Rpim	0.026	0.013	0.339
Total number of observations	103121	1773	11157
Number of reflections	25975
<I/σ(I)>	14.5	37.7	2.2
Completeness [%]	99.5	95.3	99.4
Redundancy	4	3.6	4.1
CC(1/2)	0.999	0.999	0.814

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.1 M HEPES, pH 7.5, 0.1 M NaCl, 1.6 M ammonium sulfate