5BPI
Structure of TrmBL2, an archaeal chromatin protein, shows a novel mode of DNA binding.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.062690 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.764, 154.379, 176.123 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.394 - 3.198 |
| R-factor | 0.2569 |
| Rwork | 0.255 |
| R-free | 0.29880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5bpd |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.518 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.312 |
| High resolution limit [Å] | 3.198 | 3.198 |
| Rmerge | 0.382 | |
| Number of reflections | 27256 | |
| <I/σ(I)> | 7.8 | 0.99 |
| Completeness [%] | 100.0 | 98 |
| Redundancy | 13.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 0.1M Imidazole HCl, pH 8.0, 30% MPD and 10% PEG4000 |
