5BNV

Crystal structure of Human MCM2 HBD chaperoning a histone H3-H4 tetramer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.075
Spacegroup name	P 41 21 2
Unit cell lengths	110.592, 110.592, 203.265
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.056 - 2.795
R-factor	0.1974
Rwork	0.196
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.212
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	97.144	2.950
High resolution limit [Å]	2.795	2.795
Number of reflections	31946
<I/σ(I)>	29.7	2
Completeness [%]	100.0	100
Redundancy	14.1	13.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	1.5 M sodium/potassium phosphate, pH 8.0