5BNB
Crystal structure of a Ube2S-ubiquitin conjugate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-07-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97945 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 94.906, 45.854, 110.576 |
Unit cell angles | 90.00, 93.14, 90.00 |
Refinement procedure
Resolution | 47.382 - 2.490 |
R-factor | 0.2462 |
Rwork | 0.243 |
R-free | 0.30400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zdn |
RMSD bond length | 0.002 |
RMSD bond angle | 0.629 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.380 | 2.590 |
High resolution limit [Å] | 2.490 | 2.490 |
Rmerge | 0.056 | |
Number of reflections | 32942 | |
<I/σ(I)> | 16.3 | 2.2 |
Completeness [%] | 97.4 | 95.3 |
Redundancy | 3.2 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1M Tris, 20% PEG 4000 |