5BN8
Crystal structure of nucleotide-free human Hsp70 NBD.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.129, 63.700, 144.118 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.350 - 1.340 |
| R-factor | 0.132 |
| Rwork | 0.131 |
| R-free | 0.15400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3atv |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.998 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP (11.0) |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.350 | 1.390 |
| High resolution limit [Å] | 1.340 | 1.340 |
| Rmerge | 0.038 | 0.475 |
| Number of reflections | 93866 | |
| <I/σ(I)> | 17.85 | 2.48 |
| Completeness [%] | 97.3 | 81.8 |
| Redundancy | 3.9 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 25 % PEG-3350 W/V, 0.1 M HEPES |
